Welcome to Homepage of Professor Dr. Huangxian Ju
 
English
Main page

Research Fields:

  Current research interests   are in analytical   biochemistry, molecular   diagnosis, biosensors and   chips, clinic immunoassay,   cell analytical chemistry,   nanotechnology,   electroanalysis,   luminescence analysis,   capillary electrophoresis,   and applications of PCR   technology.

Highlight works               

    Key Laboratory of Analytical     Chemistry for Life Science     (Ministry of Education of China)

   Department of Chemistry     Nanjing University 

   Nanjing 210093, P.R.China;   

   Tel: +86-25-83593593 (O)  

   Fax: +86-25-83593593   

   E-mail: hxju@nju.edu.cn

 

  

Interesting links :

The h-index of Professor Ju is 51.

In 1997 this group introduced nanotechnology to biosensing field as one of the earliest groups, and then proposed a series of novel methods for studying the direct electrochemistry of proteins and preparing novel electrochemical biosensors, which have been extensively applied and cited by SCI-journal papers. In 2000 this group combined for the first time PCR technology with electroanalytical chemistry and prepared several highly sensitive DNA biosensors. They then developed the electrochemical cytosensing field, presented the methods for exogenous effect study of cell viability and electrochemical antitumor drug sensitivity test, and proposed the first ECL biosensor based on quantum dots for the first application in bioanalysis in 2003-2005. In 2005-2008 they presented five strategies for chemiluminescent multiplex immunoassay. After 2007, they presented a new ECL mechanism based on the energy transfer of quantum dots for sensitive biosensing and proposed a creative method for fracture sampling on silicone/quartz capillary microchip, designed a method for facile synthesis of highly luminescent CdTe QDs in aqueous solution and tried to develop some protocols for in-situ monoring of cell surface carbohydrates. The current research fields in this group include bioanalytical chemistry, biosensors and chips, nanobiotechnology and molecular diagnosis. The main contributions of this group can be summarized as follows:

1) Cytosensing and molecular diagnostics. Several novel protocols based on a surface-confined carbohydrate strategy (J. Am. Chem. Soc. 2008, 130, 7224) and dual signal amplification with tetrapeptide functionalized carbon nanotubes for convenient in situ evaluation of cell surface carbohydrate sites of interest were presented. A series of methods for monitoring of cell adhesion, exogenous effect study of cell viability and electrochemical antitumor drug sensitivity test were developed. Some electrochemical hybridization biosensors with detection limits down to 1.7กม10-20 mol DNA were designed for detection of short-stranded HBV DNA by combining a PCR procedure.

2) Bioanalysis based on electrochemiluminescence of quantum dots. Their works brought a new field in application of quantum dots. The first ECL sensor for H2O2 and the first ECL biosensor for glucose based on the intrinsic ECL of quantum dots were proposed. An electrogenerated precursor was firstly designed for facile synthesis of highly luminescent QDs in aqueous solution, and several methods for ECL detection of biomolecules, including the first coreactant for extremely sensitive anodic ECL of QDs, were developed.

3) Immunosensors and immunoassay. They widened the application of immunoassay by preparing a series of novel amperometric immunosensors and their arrays for development of new immunoassay and clinic diagnostic methods. Especially, an electric field-driven strategy, two accelerating strategies for fast multiplexed detection of protein biomarkers, four new resolution strategies for chemiluminescent multiplex immunoassay and several relevant detection systems and an instrument were presented for early screening of tumors.

4) Nanobiotechnology and nanoanalysis. Two facile methods for preparation of surface protein-imprinted nanowires and nanoparticles were developed for protein specific recognition. Several novel nanocomposites, nanoparticles films, nanocage, conductive ormosil materials, functionalized ordered nanoporous silica and protein-nanoparticles architecture were prepared for preparation of novel biosensors. These materials greatly improve the performance of biosensors for different biomolecules.

Up to now, this group has published 400 papers (350 in SCI journals, including 227 papers in journals with IF>3.0 and 129 in journals with IF>5.0), especially, 1 paper in J. Am. Chem. Soc., 36 papers in Anal. Chem., 3 in Angew. Chem. Int. Ed., 2 in Adv. Mater., 5 in Adv. Funct. Mater., 2 in Clin. Chem. 37 in Biosens. Bioelectron and 1 in Clin. Cancer Res. 297 papers have been cited by SCI journals for 9114 times, including 8185 times by other authors, the average citation is 26.13 , and the h-index is 53.

Friendly links

 

Copyright @ Research Group of Analytical Biochemistry and Molecule Diagnostics, maintained by Dr H.X. Ju on August 16, 2012